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KMID : 1140120110160040302
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Cancer Prevention Research
2011 Volume.16 No. 4 p.302 ~ p.310
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Induced Apoptosis in HeLa Human Cervical Adenocarcinoma by the Extract of the Marine Algae Symbiotic Fungus Chaetomium sp.
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Jo Mi-Jeong
Son Byeong-Wha
Kim Gun-Do
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Abstract
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Natural compounds from marine organisms have been reported to possess several biological activities for the anti-oxidation, anti-diabetes, anti-inflammation and chemo-prevention against several vascular diseases. In this study, the anti-cancer effects of the extract of marine algae symbiotic fungus Chaetomium sp. on human cervical adenocarcinoma (HeLa) cells were investigated. Treatment of the extract on HeLa cells inhibited the cell proliferation and viability (IC50: 0.5μM) in a dose-dependent manner. Morphological observation revealed round-up shape and cells were finally floating. Also, the extract induced the activation of caspase-3, -6, -7, ?8 and the cleavage of poly (ADP-ribose) polymerase (PARP). The substantial increase in positively stained cleaved caspase-3 and PARP with nuclear condensation (detected by DAPI staining) indicated the induction of apoptotic cell death with the involvement of activated caspase-8. The extract also induced cell cycle arrest with the up and down-regulation of p21waf1/cip1 and phospho-Cdc2 (Tyr15), respectively. The results suggested that the marine microbial extract has a potential in cancer prevention by the inhibition of cell proliferation and the induction of apoptotic cell death.
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KEYWORD
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Marine algae symbiotic fungus, Chaetomium sp., Apoptosis, Cell cycle, HeLa
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